Walker Downey & Associates, Inc.
Plasmid Vector Design
Biotechnology has provided the means to mass produce proteins that either don’t exist or aren’t highly abundant in nature. The proteins of biotechnology have found use as means for treating life-long illness such as diabetes, and as novel therapies to combat acute illnesses such as cancer. One key to producing such valuable proteins is the construction of efficient expression plasmids that produce maximal protein yields in the cells of your choice. Walker Downey and Associates have over 20 years of experience in designing, testing, and using expression plasmids in mammalian, plant, bacterial, insect and yeast cells to produce recombinant proteins. Read the boxes below to see how we may assist you in your recombinant protein work.
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Custom Expression Plamids |
Working with a client’s scientists and intellectual property lawyers, Walker Downey and Associates will develop novel expression plasmids that produce high yields of recombinant proteins and are free from infringing intellectual property concerns. We will produce a series of plasmids with different regulatory elements (promoters, introns, untranslated regions, polyadenylation regions, etc) that perform well in your cell type and may be patented, thus protecting this key component of your production system.
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Tailoring Client Supplied Plasmids |
Working from a client supplied plasmid, Walker Downey and Associates will customize the plasmid to function as a discovery tool. The possibilities are endless, but examples include tailoring the plasmid to accept mRNA pools creating expression libraries, or to allow interchangeability of regulatory elements within a plasmid, useful for optimizing expression of new target proteins by promoter screening.
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Recombinant Services
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The construction of expression plasmids is near the front of the product pipeline. Many candidate proteins are needed to fill the pipeline because there are few viable candidates that emerge from the pipeline’s end. Walker Downey and Associates can produce the expression plasmids a client needs to feed the pipeline, freeing the client’s staff to pursue the downstream identification and characterization of lead candidates. |
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Troubleshooting
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Sometimes expression plasmids just don’t work as well as hoped. Walker Downey and Associates scientists will perform computer analyses and literature searches to find reasons a client’s plasmids aren’t performing, and provide detailed suggestions for getting the plasmid to perform up to expectations.
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